Product Info Summary
SKU: | A03462-1 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Mbd4 Antibody Picoband™
SKU/Catalog Number
A03462-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-Mbd4 Antibody Picoband™ catalog # A03462-1. Tested in WB, FCM, ELISA applications. This antibody reacts with Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Mbd4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03462-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Clone Number
1B9
Isotype
IgG
Immunogen
E.coli-derived mouse Mbd4 recombinant protein (Position: R238-D534). Mouse Mbd4 shares 69.7% amino acid (aa) sequence identity with human MBD4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A03462-1 is reactive to MBD4 in Mouse, Rat
Applications
A03462-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
66 kDa
Calculated molecular weight
66.051kDa
Background of MBD4
Methyl-CpG-binding domain protein 4 is a protein that in humans is encoded by the MBD4 gene. It is mapped to 3q21.3. The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains an MBD domain at the N-terminus that functions both in binding to methylated DNA and in protein interactions and a C-terminal mismatch-specific glycosylase domain that is involved in DNA repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Mouse
ELISA, 0.1-0.5 µg/ml, Mouse
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Mbd4 using anti-Mbd4 antibody (A03462-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mbd4 antigen affinity purified polyclonal antibody (Catalog # A03462-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mbd4 at approximately 66 kDa. The expected band size for Mbd4 is at 63 kDa.
Click image to see more details
Figure 2. Flow Cytometry analysis of C2C12 cells using anti-Mbd4 antibody (A03462-1).
Overlay histogram showing C2C12 cells stained with A03462-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Mbd4 Antibody (A03462-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For MBD4 (Source: Uniprot.org, NCBI)
Gene Name
MBD4
Full Name
Methyl-CpG-binding domain protein 4
Weight
66.051kDa
Alternative Names
EC 3.2.2.-; G/T mismatch glycosylase; G/U mismatch glycosylase; MBD4; MED1G/5-fluorouracil mismatch glycosylase with biphasic kinetics; methyl-CpG binding domain protein 4; methyl-CpG-binding domain protein 43,N(4)-ethenocytosine glycosylase; Methyl-CpG-binding endonuclease 1; Methyl-CpG-binding protein MBD4; Mismatch-specific DNA N-glycosylase; putative methyl-CpG binding protein MBD4 MED1 methyl-CpG binding domain 4, DNA glycosylase methyl-CpG-binding domain protein 4|3,N(4)-ethenocytosine glycosylase|G/5-fluorouracil mismatch glycosylase with biphasic kinetics|G/T mismatch glycosylase|G/U mismatch glycosylase|methyl-CpG binding domain protein 4|methyl-CpG-binding endonuclease 1|methyl-CpG-binding protein MBD4|mismatch-specific DNA N-glycosylase|putative methyl-CpG binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MBD4, check out the MBD4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MBD4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Mbd4 Antibody Picoband™ (A03462-1)
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No publications found for A03462-1
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